BCOX1 Rabbit pAb

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BCOX1) Polyclonal Antibody, Unconjugated (AP56481) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF
Primary Accession	Q14667
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	253700 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human BCOX1
Epitope Specificity	131-230/2235
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Secreted.
SIMILARITY	Belongs to the UPF0378 family.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	KIAA0100 (KIAA0100) is a Protein Coding gene. Diseases associated with KIAA0100 include Monocytic Leukemia and In Situ Carcinoma. May be involved in membrane trafficking.

Additional Information
Gene ID	9703
Other Names	Bridge-like lipid transfer protein family member 2, Antigen MLAA-22, Breast cancer-overexpressed gene 1 protein, Protein hobbit homolog, BLTP2 (HGNC:28960), BCOX1, KIAA0100
Target/Specificity	Expressed in pancreas, placenta and up-regulated in breast carcinoma epithelial cells, ductal in situ carcinoma (DCIS), invasive breast carcinoma (IBC) and metastatic breast carcinoma cells (MET).
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	BLTP2 (HGNC:28960)
Synonyms	BCOX1, KIAA0100
Function	Tube-forming lipid transport protein which binds to phosphatidylinositols and affects phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P2) distribution.
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:Q9VZS7}. Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:Q9VZS7} Mitochondrion membrane {ECO:0000250\|UniProtKB:Q06179}. Note=Localizes to endoplasmic reticulum-cell membrane and some endoplasmic reticulum- mitochondria contact sites. {ECO:0000250\|UniProtKB:Q9VZS7}
Tissue Location	Expressed in pancreas, placenta and up-regulated in breast carcinoma epithelial cells, ductal in situ carcinoma (DCIS), invasive breast carcinoma (IBC) and metastatic breast carcinoma cells (MET).

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

KIAA0100 (KIAA0100) is a Protein Coding gene. Diseases associated with KIAA0100 include Monocytic Leukemia and In Situ Carcinoma. May be involved in membrane trafficking.

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¥ 1,500.00

Cat# AP56481

Size:

50 µl

100 µl

Price:

¥ 1,500.00

¥ 2,600.00

Quantity:

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Availability: 3-5 days

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BCOX1 Rabbit pAb

BCOX1 Rabbit pAb

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