LMAN1L Rabbit pAb

Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-LMAN1L (AP57030) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LMAN1L) Polyclonal Antibody, Unconjugated (AP57030) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q9HAT1
Reactivity	Mouse
Predicted	Human, Rat, Dog, Pig, Horse, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	57129 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human LMAN1L
Epitope Specificity	26-130/526
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Additional Information
Gene ID	79748
Other Names	Protein ERGIC-53-like, ERGIC53-like protein, Lectin mannose-binding 1-like, LMAN1-like protein, LMAN1L, ERGL
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	LMAN1L
Synonyms	ERGL
Cellular Location	Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein
Tissue Location	Highly expressed in normal and neoplastic prostate. Also expressed in cardiac atrium, salivary gland, spleen and selective cells in the CNS.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.