Nucleobindin 1 Rabbit pAb

Sample:
A673(Human) Cell Lysate at 30 ug
Primary: Anti-alpha smooth muscle Actin (AP57550) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nucleobindin 1) Polyclonal Antibody, Unconjugated (AP57550) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q02818
Reactivity	Rat, Human, Mouse
Predicted	Pig, Rabbit, Dog, Horse, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	53879 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human Nucleobindin 1
Epitope Specificity	21-120/461
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Golgi apparatus > cis-Golgi network membrane. Cytoplasm. A small fraction of the protein may be cytoplasmic.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq, Jun 2010]

Additional Information
Gene ID	4924
Other Names	Nucleobindin-1, CALNUC, NUCB1, NUC
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	NUCB1
Synonyms	NUC
Function	Major calcium-binding protein of the Golgi which may have a role in calcium homeostasis (By similarity). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates alpha subunits of guanine nucleotide-binding proteins (G proteins) (By similarity).
Cellular Location	Golgi apparatus, cis-Golgi network membrane {ECO:0000250\|UniProtKB:Q63083}; Peripheral membrane protein {ECO:0000250\|UniProtKB:Q63083}; Lumenal side {ECO:0000250\|UniProtKB:Q63083}. Cytoplasm {ECO:0000250\|UniProtKB:Q63083}. Secreted {ECO:0000250\|UniProtKB:Q63083}. Note=A small fraction of the protein may be cytoplasmic. {ECO:0000250\|UniProtKB:Q63083}
Tissue Location	Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.