SEZ6L Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- SEZ6L (AP58377) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 109 kD
Observed band size: 110 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SEZ6L) Polyclonal Antibody, Unconjugated (AP58377) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, E
Primary Accession	Q9BYH1
Reactivity	Rat, Pig, Dog, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	111782 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human SEZ6L
Epitope Specificity	701-800/1024
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Endoplasmic reticulum membrane; Single-pass type I membrane protein
SIMILARITY	Belongs to the SEZ6 family. Contains 3 CUB domains. Contains 5 Sushi (CCP/SCR) domains.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	SEZ6L is a candidate tumor suppressor gene. This protein may contribute to specialized endoplasmic reticulum functions in neurons.

Additional Information
Gene ID	23544
Other Names	Seizure 6-like protein, SEZ6L, KIAA0927
Target/Specificity	Widely expressed, including adult and fetal brains and lungs. Not expressed in all lung cancer cell lines.
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	SEZ6L
Synonyms	KIAA0927
Function	May contribute to specialized endoplasmic reticulum functions in neurons.
Cellular Location	Endoplasmic reticulum membrane; Single-pass type I membrane protein
Tissue Location	Widely expressed, including adult and fetal brains and lungs. Not expressed in all lung cancer cell lines

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.