SCGB3A1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCGB3A1) Polyclonal Antibody, Unconjugated (AP58437) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF, E
Primary Accession	Q96QR1
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	10100 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human SCGB3A1
Epitope Specificity	51-104/104
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Secreted.
SIMILARITY	Belongs to the secretoglobin family. UGRP subfamily.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	SCGB3A1 is a potential growth inhibitory cytokine.

Additional Information
Gene ID	92304
Other Names	Secretoglobin family 3A member 1, Cytokine HIN-1, High in normal 1, Pneumo secretory protein 2, PnSP-2, Uteroglobin-related protein 2, SCGB3A1, HIN1, PNSP2, UGRP2
Target/Specificity	Highly expressed in breast tissues. Absent in breast cancer cell lines.
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	SCGB3A1
Synonyms	HIN1, PNSP2, UGRP2
Function	Secreted cytokine-like protein. Inhibits cell growth in vitro.
Cellular Location	Secreted.
Tissue Location	Highly expressed in lung and prostate (PubMed:11481438). Also found in mammary gland, spleen, pancreas, testis and liver (PubMed:11481438). Detected throughout the airway epithelium in lung, with highest expression in large airways (PubMed:12406855). Found in lung submucosal glands where it localizes to acinar and ductile cells (PubMed:12406855). Not detected in respiratory bronchioles, alveolar ducts or alveolar epithelium (PubMed:12406855). In mammary gland, specifically localizes to luminal epithelial cells (PubMed:11481438).

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.