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Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-RASGRP1 Antibody (Center) 1:1000 dilution Lane 1: A549 whole cel lysate Lane 2: SW480 whole cel lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 100kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-RASGRP1 Antibody (Center) 1:1000 dilution Lane 1: A549 whole cel lysate Lane 2: SW480 whole cel lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 100kDa Blocking/Dilution buffer: 5% NFDM/TBST.
RASGRP1 Antibody (Center) (Cat. #AP5856c) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RASGRP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
RASGRP1 Antibody (Center) (Cat. #AP5856c) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	O95267
Other Accession	Q9R1K8, Q9Z1S3, NP_005730.2, NP_001122074.1
Reactivity	Human
Predicted	Mouse, Rat, Bovine, Canine, Rabbit
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	90402 Da
Antigen Region	495-521 aa

Additional Information
Gene ID	10125
Other Names	RAS guanyl-releasing protein 1, Calcium and DAG-regulated guanine nucleotide exchange factor II, CalDAG-GEFII, Ras guanyl-releasing protein, RASGRP1, RASGRP
Target/Specificity	This RASGRP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495-521 amino acids from the Central region of human RASGRP1.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	RASGRP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	RASGRP1
Synonyms	RASGRP
Function	Functions as a calcium- and diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP (PubMed:15899849, PubMed:23908768, PubMed:27776107, PubMed:29155103). Activates the Erk/MAP kinase cascade (PubMed:15899849). Regulates T-cell/B-cell development, homeostasis and differentiation by coupling T-lymphocyte/B-lymphocyte antigen receptors to Ras (PubMed:10807788, PubMed:12839994, PubMed:27776107, PubMed:29155103). Regulates NK cell cytotoxicity and ITAM-dependent cytokine production by activation of Ras-mediated ERK and JNK pathways (PubMed:19933860). Functions in mast cell degranulation and cytokine secretion, regulating FcERI-evoked allergic responses. May also function in differentiation of other cell types (PubMed:12845332).
Cellular Location	Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein Note=Found both in the cytosol and associated with membranes Relocalization to the cell membrane upon activation is F-actin- dependent. Translocates to the Golgi in response to phorbol ester or nerve growth factor. Localizes to somata and dendrites but not to axons of hippocampal pyramidal cells (By similarity).
Tissue Location	Expressed in brain with higher expression in cerebellum, cerebral cortex and amygdala. Expressed in the hematopoietic system. Expressed in T-cells (at protein level) Expressed in NK cells (at protein level) (PubMed:19933860)