SLC12A5 Antibody (N-term) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-SLC12A5 Antibody (N-term) at 1:4000 dilution + human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 126 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
SLC12A5 Antibody (N-term) (Cat. #AP5876a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SLC12A5 antibody detected the SLC12A5 protein (arrow).
SLC12A5 Antibody (N-term) (Cat. #AP5876a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC12A5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q9H2X9
Other Accession	NP_065759.1, NP_001128243.1
Reactivity	Human
Predicted	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	126184 Da
Antigen Region	5-33 aa

Additional Information
Gene ID	57468
Other Names	Solute carrier family 12 member 5, Electroneutral potassium-chloride cotransporter 2, K-Cl cotransporter 2, hKCC2, Neuronal K-Cl cotransporter, SLC12A5, KCC2, KIAA1176
Target/Specificity	This SLC12A5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-33 amino acids from the N-terminal region of human SLC12A5.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	SLC12A5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SLC12A5 (HGNC:13818)
Function	Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis (PubMed:12106695). As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition (By similarity). Involved in the regulation of dendritic spine formation and maturation (PubMed:24668262).
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:Q91V14}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:Q91V14}. Cell projection, dendrite {ECO:0000250\|UniProtKB:Q91V14}. Note=Detected on dendrites, but not on axons of spinal cord neurons and at GPHN-positive inhibitory synapses. {ECO:0000250\|UniProtKB:Q91V14}
Tissue Location	Brain specific. Detected in neuronal cells.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.