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ESRP1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ESRP1 Antibody  (N-term) AP5940a
    All lanes : Anti-ESRP1 Antibody (N-term) at 1:1000 dilution Lane 1: HACAT whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Observed band size :72kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - ESRP1 Antibody  (N-term) AP5940a
    ESRP1 antibody (N-term) (Cat. #AP5940a) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ESRP1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q6NXG1
Other Accession B2RYD2, Q3US41, NP_001116298.1, NP_060167.2, NP_001030087.2, NP_001116299.1, NP_001116297.1
Reactivity Human, Rat, Mouse
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 75585 Da
Antigen Region 182-211 aa
Additional Information
Gene ID 54845
Other Names Epithelial splicing regulatory protein 1, RNA-binding motif protein 35A, RNA-binding protein 35A, ESRP1, RBM35A
Target/Specificity This ESRP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-211 amino acids of human ESRP1.
Dilution WB~~1:1000
IHC-P~~1:100~500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsESRP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ESRP1
Synonyms RBM35A
Function mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2 (PubMed:19285943). Regulates splicing and expression of genes involved in inner ear development, auditory hair cell differentiation, and cell fate specification in the cochlear epithelium (By similarity).
Cellular Location Nucleus.
Tissue Location Epithelial cell-specific.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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