Anti-BRS3 Antibody

Western blot analysis of BRS3 expression in A549 (A), H9C2 (B), PC3 (C), Panc1 (D) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 50 kD)
Immunofluorescent analysis of BRS3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P32247
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	44411 Da

Additional Information
Gene ID	680
Other Names	Bombesin receptor subtype-3; BRS-3
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BRS3. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	BRS3
Function	Role in sperm cell division, maturation, or function. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Cellular Location	Cell membrane; Multi-pass membrane protein.
Tissue Location	In germ cells in testis. Lung carcinoma cells.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.