Anti-FYB Antibody

Western blot analysis of FYB expression in H9C2 (A), SP20 (B), PC3 (C) whole cell lysates. (Predicted band size: 85 kD; Observed band size: 110 kD)
Immunohistochemical analysis of FYB staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of FYB staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	O15117
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	85387 Da

Additional Information
Gene ID	2533
Other Names	SLAP130; FYN-binding protein; Adhesion and degranulation promoting adaptor protein; ADAP; FYB-120/130; p120/p130; FYN-T-binding protein; SLAP-130; SLP-76-associated phosphoprotein
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human FYB. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	FYB1 (HGNC:4036)
Synonyms	FYB, SLAP130
Function	Acts as an adapter protein of the FYN and LCP2 signaling cascades in T-cells (By similarity). May play a role in linking T-cell signaling to remodeling of the actin cytoskeleton (PubMed:10747096, PubMed:16980616). Modulates the expression of IL2 (By similarity). Involved in platelet activation (By similarity). Prevents the degradation of SKAP1 and SKAP2 (PubMed:15849195). May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells (By similarity).
Cellular Location	Cytoplasm. Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00768}. Cell junction {ECO:0000250\|UniProtKB:O35601}. Note=Colocalizes with TMEM47 at cell- cell contacts in podocytes. {ECO:0000250\|UniProtKB:O35601}
Tissue Location	Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non- lymphoid tissues