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Anti-SMAD6 Antibody

Rabbit polyclonal antibody to SMAD6

     
  • 1 - Anti-SMAD6 Antibody AP59616
    Western blot analysis of SMAD6 expression in A549 (A), PC3 (B), CT26 (C), H9C2 (D) whole cell lysates.
  • 2 - Anti-SMAD6 Antibody AP59616
    Immunohistochemical analysis of SMAD6 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession O43541
Other Accession O35182
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 53497 Da
Additional Information
Gene ID 4091
Other Names MADH6; Mothers against decapentaplegic homolog 6; MAD homolog 6; Mothers against DPP homolog 6; SMAD family member 6; SMAD 6; Smad6; hSMAD6
Target/Specificity Recognizes endogenous levels of SMAD6 protein.
Dilution WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name SMAD6
Synonyms MADH6
Function Transforming growth factor-beta superfamily receptors signaling occurs through the Smad family of intracellular mediators. SMAD6 is an inhibitory Smad (i-Smad) that negatively regulates signaling downstream of type I transforming growth factor-beta (PubMed:10647776, PubMed:10708948, PubMed:10708949, PubMed:16951688, PubMed:22275001, PubMed:30848080, PubMed:9436979, PubMed:9759503). Acts as a mediator of TGF-beta and BMP anti-inflammatory activities. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B- mediated expression of pro-inflammatory genes (PubMed:16951688). Blocks the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor- activated SMAD1-binding (PubMed:30848080, PubMed:9436979). Binds to regulatory elements in target promoter regions (PubMed:16491121).
Cellular Location Nucleus.
Tissue Location [Isoform B]: Expressed in the brain, heart, ovary, peripheral blood leukocytes, small intestine, spleen, thymus, bone marrow, fetal liver and lymph nodes.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SMAD6. The exact sequence is proprietary.

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