Anti-MMP23 Antibody

Western blot analysis of MMP23 expression in mouse heart (A), mouse muscle (B), rat heart (C), rat muscle (D) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 43; 54 kD)
Immunofluorescent analysis of MMP23 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	O75900
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	43935 Da

Additional Information
Gene ID	8510
Other Names	MMP21; MMP21; MMP22; Matrix metalloproteinase-23; MMP-23; Femalysin; MIFR-1; Matrix metalloproteinase-21; MMP-21; Matrix metalloproteinase-22; MMP-22
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MMP23. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MMP23B
Synonyms	MMP21, MMP22
Function	Protease. May regulate the surface expression of some potassium channels by retaining them in the endoplasmic reticulum (By similarity).
Cellular Location	Endoplasmic reticulum membrane; Single-pass type II membrane protein. Membrane; Single-pass type II membrane protein. Note=A secreted form produced by proteolytic cleavage may also exist.
Tissue Location	Predominantly expressed in ovary, testis and prostate.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.