Anti-CSP Antibody

Western blot analysis of CSP expression in AML12 (A), PMVEC (B), A549 (C), LO2 (D), H1792 (E) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 22; 34 kD)
Immunofluorescent analysis of CSP staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q9H3Z4
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	22149 Da

Additional Information
Gene ID	80331
Other Names	CSP; DnaJ homolog subfamily C member 5; Cysteine string protein; CSP
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CSP. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	DNAJC5 (HGNC:16235)
Function	Acts as a general chaperone in regulated exocytosis (By similarity). Acts as a co-chaperone for the SNARE protein SNAP-25 (By similarity). Involved in the calcium-mediated control of a late stage of exocytosis (By similarity). May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings (By similarity).
Cellular Location	Cytoplasm, cytosol {ECO:0000250\|UniProtKB:Q29455}. Membrane {ECO:0000250\|UniProtKB:Q29455}; Lipid-anchor {ECO:0000250\|UniProtKB:Q29455}. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane {ECO:0000250\|UniProtKB:Q29455}. Melanosome. Cell membrane. Note=The association with membranes is regulated by palmitoylation (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). {ECO:0000250\|UniProtKB:Q29455, ECO:0000269\|PubMed:17081065}
Tissue Location	Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.