Anti-Cyclin E1 (pT395) Antibody

Rabbit polyclonal antibody to Cyclin E1 (pT395)

Western blot analysis of Cyclin E1 (pT395) expression in mouse kidney (A), rat kidney (B) whole cell lysates.
Immunohistochemical analysis of Cyclin E1 (pT395) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P24864
Other Accession	Q61457
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	47077 Da

Additional Information
Gene ID	898
Other Names	CCNE; G1/S-specific cyclin-E1
Target/Specificity	Recognizes endogenous levels of Cyclin E1 (pT395) protein.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	CCNE1
Synonyms	CCNE
Function	Essential for the control of the cell cycle at the G1/S (start) transition.
Cellular Location	Nucleus.
Tissue Location	Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

Research Areas