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Anti-CRHR1 Antibody

Western blot analysis of CRHR1 expression in HEK293T (A), Hela (B), DLD (C) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 66 kD)
Immunofluorescent analysis of CRHR1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P34998
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	47671 Da

Additional Information
Gene ID	1394
Other Names	CRFR; CRFR1; CRHR; Corticotropin-releasing factor receptor 1; CRF-R-1; CRF-R1; CRFR-1; Corticotropin-releasing hormone receptor 1; CRH-R-1; CRH-R1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CRHR1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CRHR1 (HGNC:2357)
Function	G protein-coupled receptor for CRH and UCN stress hormones, which plays a central role in whole body adaptation to stress (PubMed:18292205, PubMed:18801728, PubMed:20966082, PubMed:23576434, PubMed:23863939, PubMed:24290358, PubMed:32004469, PubMed:32004470). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and downstream effectors, such as adenylate cyclase (PubMed:18292205, PubMed:32004469, PubMed:32004470). CRHR1 is mainly coupled to G(s) G protein (GNAS), mediating activation of adenylate cyclase activity and production of cAMP (PubMed:18292205, PubMed:32004469, PubMed:32004470). In some conditions, can also couple to G(q) G protein (GNAQ) and mediate production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers (PubMed:14657255). CRHR1-dependent signaling is a primary mediator of the neuroendocrine, autonomic (fight-or-flight) and behavioral responses to stress, acting as a key regulator of adaptation by activating the hypothalamus-pituitary-adrenal axis, leading to corticotropin hormone (ACTH) production (By similarity). Mediates both anxiogenic and anxiolytic response to stress in glutamatergic and dopaminergic neurons, respectively (By similarity). CRHR1 also plays a critical role in addiction by mediating the brain's stress response, particularly during withdrawal and relapse: it drives the transition to compulsive drug seeking by increasing anxiety and negative emotional states (By similarity).
Cellular Location	Cell membrane; Multi-pass membrane protein. Note=Agonist-binding promotes endocytosis.
Tissue Location	Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe