Anti-CD137L Antibody

Western blot analysis of CD137L expression in HEK293T (A), H1688 (B), A549 (C) whole cell lysates. (Predicted band size: 26 kD; Observed band size: 70 kD)
Immunofluorescent analysis of CD137L staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P41273
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	26625 Da

Additional Information
Gene ID	8744
Other Names	Tumor necrosis factor ligand superfamily member 9; 4-1BB ligand; 4-1BBL
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD137L. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TNFSF9
Function	Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation- induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.
Cellular Location	Membrane; Single-pass type II membrane protein.
Tissue Location	Expressed in brain, placenta, lung, skeletal muscle and kidney

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.