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Anti-PICK1 Antibody

Western blot analysis of PICK1 expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46; 52 kD)
Immunofluorescent analysis of PICK1 staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q9NRD5
Reactivity	Human, Mouse, Rat, Bovine, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	46600 Da

Additional Information
Gene ID	9463
Other Names	PRKCABP; PRKCA-binding protein; Protein interacting with C kinase 1; Protein kinase C-alpha-binding protein
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PICK1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	PICK1
Synonyms	PRKCABP
Function	Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competitive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor (AMPAR) endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression (LTD). Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function.
Cellular Location	Cytoplasm, perinuclear region {ECO:0000250\|UniProtKB:Q9EP80}. Membrane {ECO:0000250\|UniProtKB:Q9EP80}; Peripheral membrane protein {ECO:0000250\|UniProtKB:Q9EP80}. Membrane {ECO:0000250\|UniProtKB:Q62083}; Lipid-anchor {ECO:0000250\|UniProtKB:Q62083}. Postsynaptic density {ECO:0000250\|UniProtKB:Q9EP80}. Synapse, synaptosome {ECO:0000250\|UniProtKB:Q9EP80}. Cytoplasm, cytoskeleton {ECO:0000250\|UniProtKB:Q9EP80}. Note=Also membrane-associated, present at excitatory synapses. {ECO:0000250\|UniProtKB:Q9EP80}
Tissue Location	Ubiquitous.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol