Anti-Septin 8 Antibody

Western blot analysis of Septin 8 expression in rat brain (A) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 55 kD)
Immunohistochemical analysis of Septin 8 staining in human lymphoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Septin 8 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	Q92599
Reactivity	Human, Mouse, Rat, Rabbit, Bovine, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	55756 Da

Additional Information
Gene ID	23176
Other Names	KIAA0202; Septin-8
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Septin 8. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SEPTIN8 (HGNC:16511)
Function	Filament-forming cytoskeletal GTPase (By similarity). May play a role in platelet secretion (PubMed:15116257). Seems to participate in the process of SNARE complex formation in synaptic vesicles (By similarity).
Cellular Location	Cytoplasm {ECO:0000250\|UniProtKB:B0BNF1}. Cytoplasm, cytoskeleton. Synapse {ECO:0000250\|UniProtKB:B0BNF1}. Cell projection, axon {ECO:0000250\|UniProtKB:B0BNF1}. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250\|UniProtKB:B0BNF1}. Presynapse {ECO:0000250\|UniProtKB:B0BNF1}. Note=Expressed in axons of immature neurons, localizes to synapses in mature neurons (By similarity). In platelets, found in areas surrounding alpha-granules (PubMed:15116257) {ECO:0000250\|UniProtKB:B0BNF1, ECO:0000269\|PubMed:15116257}
Tissue Location	Widely expressed, including in brain, heart and platelets; most abundant in aorta. Isoform 2 is expressed at low levels in specific brain areas, such as occipital pole, frontal lobe, temporal lobe and putamen. Isoform 1 and 3 are highly expressed in specific brain areas, such as occipital pole, frontal lobe, temporal lobe and putamen. Isoform 2 is highly expressed in prostate, testis and ovary Isoform 1 and isoform 3 are expressed at low levels in prostate, testis and ovary.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.