Anti-ACC alpha (Phospho-S80) Antibody

Western blot analysis of ACC alpha (Phospho-S80) expression in HEK293T (A) whole cell lysates. (Predicted band size: 265 kD; Observed band size: 280 kD)
Immunohistochemical analysis of ACC alpha (Phospho-S80) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of ACC alpha (pS80) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Direct ELISA antibody dose-response curve using Anti-ACC alpha (Phospho-S80) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q13085
Reactivity	Human, Mouse, Rat, Pig, Bovine, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	265554 Da

Additional Information
Gene ID	31
Other Names	ACAC; ACC1; ACCA; Acetyl-CoA carboxylase 1; ACC1; ACC-alpha
Target/Specificity	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S80 of human ACC alpha protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ACACA (HGNC:84)
Synonyms	ACAC, ACC1, ACCA
Function	Cytosolic enzyme that catalyzes the carboxylation of acetyl- CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis (PubMed:20457939, PubMed:20952656, PubMed:29899443). This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed:20457939, PubMed:20952656, PubMed:29899443).
Cellular Location	Cytoplasm, cytosol {ECO:0000250\|UniProtKB:Q5SWU9}
Tissue Location	Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver