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>   首页   >   产品   >   一抗   >   其他   >   Anti-ACC alpha (pS80) Antibody   

Anti-ACC alpha (pS80) Antibody

Rabbit polyclonal antibody to ACC alpha (pS80)

     
  • 1 - Anti-ACC alpha (pS80) Antibody AP60211
    Western blot analysis of ACC alpha (pS80) expression in HEK293T (A) whole cell lysates.
  • 2 - Anti-ACC alpha (pS80) Antibody AP60211
    Immunohistochemical analysis of ACC alpha (pS80) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • 10 - Anti-ACC alpha (pS80) Antibody AP60211
    Direct ELISA antibody dose-response curve using Anti-ACC alpha (pS80) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession Q13085
Other Accession Q5SWU9
Reactivity Human, Mouse, Rat, Pig, Bovine, SARS
Host Rabbit
Clonality Polyclonal
Calculated MW 265554 Da
Additional Information
Gene ID 31
Other Names ACAC; ACC1; ACCA; Acetyl-CoA carboxylase 1; ACC1; ACC-alpha
Target/Specificity Recognizes endogenous levels of ACC alpha (pS80) protein.
Dilution WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ACACA (HGNC:84)
Synonyms ACAC, ACC1, ACCA
Function Cytosolic enzyme that catalyzes the carboxylation of acetyl- CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis (PubMed:20457939, PubMed:20952656, PubMed:29899443). This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed:20457939, PubMed:20952656, PubMed:29899443).
Cellular Location Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q5SWU9}
Tissue Location Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ACC alpha (pS80). The exact sequence is proprietary.

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