Anti-Arylsulfatase B Antibody

Western blot analysis of Arylsulfatase B expression in Hela (A), mouse liver (B), mouse muscle (C), rat liver (D) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 60 kD)
Immunofluorescent analysis of Arylsulfatase B staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P15848
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	59687 Da

Additional Information
Gene ID	411
Other Names	Arylsulfatase B; ASB; N-acetylgalactosamine-4-sulfatase; G4S
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Arylsulfatase B. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ARSB
Function	Removes sulfate groups from chondroitin-4-sulfate (C4S) and regulates its degradation (PubMed:19306108). Involved in the regulation of cell adhesion, cell migration and invasion in colonic epithelium (PubMed:19306108). In the central nervous system, is a regulator of neurite outgrowth and neuronal plasticity, acting through the control of sulfate glycosaminoglycans and neurocan levels (By similarity).
Cellular Location	Lysosome {ECO:0000250\|UniProtKB:P50429}. Cell surface {ECO:0000250\|UniProtKB:P50429}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.