Anti-Estrogen Receptor beta Antibody

Rabbit polyclonal antibody to Estrogen Receptor beta

Western blot analysis of Estrogen Receptor beta expression in U87MG (A) whole cell lysates.
Immunofluorescent analysis of Estrogen Receptor beta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q92731
Other Accession	O08537
Reactivity	Human, Mouse, Rat, Drosophila
Host	Rabbit
Clonality	Polyclonal
Calculated MW	59216 Da

Additional Information
Gene ID	2100
Other Names	ESTRB; NR3A2; Estrogen receptor beta; ER-beta; Nuclear receptor subfamily 3 group A member 2
Target/Specificity	Recognizes endogenous levels of Estrogen Receptor beta protein.
Dilution	WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	ESR2
Synonyms	ESTRB, NR3A2
Function	Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen- dependent manner (PubMed:20074560).
Cellular Location	Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00407, ECO:0000269\|PubMed:19126643, ECO:0000269\|PubMed:20074560}
Tissue Location	[Isoform 1]: Expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. [Isoform 4]: Expressed in the testis. [Isoform 6]: Expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.