Anti-PPP2R5D Antibody

Rabbit polyclonal antibody to PPP2R5D

Western blot analysis of PPP2R5D expression in HEK293 (A), LOVO (B), mouse brain (C), mouse kidney (D) whole cell lysates.
Immunohistochemical analysis of PPP2R5D staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q14738
Reactivity	Human, Mouse, Monkey, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	69992 Da

Additional Information
Gene ID	5528
Other Names	Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform; PP2A B subunit isoform B'-delta; PP2A B subunit isoform B56-delta; PP2A B subunit isoform PR61-delta; PP2A B subunit isoform R5-delta
Target/Specificity	Recognizes endogenous levels of PPP2R5D protein.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	PPP2R5D
Function	The B regulatory subunit might modulate substrate selectivity and catalytic activity, and might also direct the localization of the catalytic enzyme to a particular subcellular compartment.
Cellular Location	Cytoplasm. Nucleus. Note=Nuclear in interphase, nuclear during mitosis
Tissue Location	Isoform Delta-2 is widely expressed. Isoform Delta- 1 is highly expressed in brain

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.