Anti-Protein C Antibody

Western blot analysis of Protein C expression in Raw264.7 (A), HepG2 (B), Jurkat (C), HEK293T (D) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 52 kD)
Immunohistochemical analysis of Protein C staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P04070
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	52071 Da

Additional Information
Gene ID	5624
Other Names	Vitamin K-dependent protein C; Anticoagulant protein C; Autoprothrombin IIA; Blood coagulation factor XIV
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Protein C. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	PROC
Function	Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265, PubMed:39880037). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).
Cellular Location	Secreted. Golgi apparatus Endoplasmic reticulum
Tissue Location	Plasma; synthesized in the liver.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.