Anti-NF-kappaB p105/p50 Antibody

Rabbit polyclonal antibody to NF-kappaB p105/p50

Western blot analysis of NF-kappaB p105/p50 expression in MCF7 (A), U2OS (B), A375 (C), mouse muscle (D), mouse liver (E) whole cell lysates.
Immunohistochemical analysis of NF-kappaB p105/p50 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P19838
Other Accession	P25799
Reactivity	Human, Mouse, Rat, Pig, Chicken, Bovine, Drosophila
Host	Rabbit
Clonality	Polyclonal

Additional Information
Other Names	Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NF-kappaB p105/p50. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt