Anti-BDKRB1 Antibody

Rabbit polyclonal antibody to BDKRB1

Western blot analysis of BDKRB1 expression in Hela (A), HepG2 (B) whole cell lysates.
Immunofluorescent analysis of BDKRB1 staining in Saos2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P46663
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	40495 Da

Additional Information
Gene ID	623
Other Names	BRADYB1; B1 bradykinin receptor; B1R; BK-1 receptor
Target/Specificity	Recognizes endogenous levels of BDKRB1 protein.
Dilution	WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	BDKRB1
Synonyms	BRADYB1
Function	This is a receptor for bradykinin. Could be a factor in chronic pain and inflammation.
Cellular Location	Cell membrane; Multi-pass membrane protein

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.