Anti-PTP alpha Antibody

Western blot analysis of PTP alpha expression in HCT116 (A), Myla2059 (B), C6 (C), CT26 (D), Hela (E) whole cell lysates. (Predicted band size: 90 kD; Observed band size: 145 kD)
Immunofluorescent analysis of PTP alpha staining in LS8 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P18433
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	90719 Da

Additional Information
Gene ID	5786
Other Names	PTPA; PTPRL2; Receptor-type tyrosine-protein phosphatase alpha; Protein-tyrosine phosphatase alpha; R-PTP-alpha
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PTP alpha. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	PTPRA
Synonyms	PTPA, PTPRL2
Function	Tyrosine protein phosphatase which is involved in integrin- mediated focal adhesion formation (By similarity). Following integrin engagement, specifically recruits BCAR3, BCAR1 and CRK to focal adhesions thereby promoting SRC-mediated phosphorylation of BRAC1 and the subsequent activation of PAK and small GTPase RAC1 and CDC42 (By similarity).
Cellular Location	Cell membrane; Single-pass type I membrane protein. Cell junction, focal adhesion {ECO:0000250\|UniProtKB:P18052}. Note=Localizes to focal adhesion sites following integrin engagement. {ECO:0000250\|UniProtKB:P18052}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.