Anti-CEP76 Antibody

Western blot analysis of CEP76 expression in COS7 (A), PC3 (B), CT26 (C), PC12 (D) whole cell lysates. (Predicted band size: 74 kD; Observed band size: 76 kD)
Immunohistochemical analysis of CEP76 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of CEP76 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q8TAP6
Reactivity	Human, Mouse, Rat, Zebrafish, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	74413 Da

Additional Information
Gene ID	79959
Other Names	C18orf9; Centrosomal protein of 76 kDa; Cep76
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CEP76. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CEP76
Synonyms	C18orf9
Function	Centrosomal protein involved in regulation of centriole duplication. Required to limit centriole duplication to once per cell cycle by preventing centriole reduplication.
Cellular Location	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Note=Does not localize along the ciliary axoneme

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.