Anti-NXF3 Antibody

Western blot analysis of NXF3 expression in H1688 (A) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 60 kD)
Immunohistochemical analysis of NXF3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of NXF3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q9H4D5
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	60102 Da

Additional Information
Gene ID	56000
Other Names	TAPL3; Nuclear RNA export factor 3; TAP-like protein 3; TAPL-3
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NXF3. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	NXF3
Synonyms	TAPL3
Function	May function as a tissue-specific nuclear mRNA export factor.
Cellular Location	Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm
Tissue Location	Expressed at high level in testis and at low level in a small number of tissues

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.