Anti-Alpha-actin Antibody

Rabbit polyclonal antibody to Alpha-actin

Western blot analysis of Alpha-actin expression in C6 (A), AML12 (B) whole cell lysates.
Immunohistochemical analysis of Alpha-actin staining in mouse skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P68032
Other Accession	P68033
Reactivity	Human, Mouse, Rat, Chicken, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	42019 Da

Additional Information
Gene ID	70
Other Names	ACTC; Actin, alpha cardiac muscle 1; Alpha-cardiac actin
Target/Specificity	Recognizes endogenous levels of Alpha-actin protein.
Dilution	WB~~WB (1/500 - 1/2000), IHC (1/50 - 1/200) IHC~~WB (1/500 - 1/2000), IHC (1/50 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	ACTC1
Synonyms	ACTC
Function	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location	Cytoplasm, cytoskeleton.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.