Anti-K Cadherin Antibody

Rabbit polyclonal antibody to K Cadherin

Western blot analysis of K Cadherin expression in HEK293T (A), C6 (B), MEF (C), HepG2 (D), H1792 (E) whole cell lysates.
Immunohistochemical analysis of K Cadherin staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P55285
Other Accession	P97326
Reactivity	Human, Mouse, Rat, Chicken, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	88309 Da

Additional Information
Gene ID	1004
Other Names	Cadherin-6; Kidney cadherin; K-cadherin
Target/Specificity	Recognizes endogenous levels of K Cadherin protein.
Dilution	WB~~WB (1/500 - 1/2000), IHC (1/50 - 1/200) IHC~~WB (1/500 - 1/2000), IHC (1/50 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	CDH6
Function	Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Highly expressed in brain, cerebellum, and kidney. Lung, pancreas, and gastric mucosa show a weak expression. Also expressed in certain liver and kidney carcinomas

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.