> 首页 > 产品 > 一抗 > 其他 > Anti-GPR66 Antibody

Anti-GPR66 Antibody

Western blot analysis of GPR66 expression in rat testis (A), mouse testis (B), SGC7901 (C), K562 (D) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD)
Immunofluorescent analysis of GPR66 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q9HB89
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	47351 Da

Additional Information
Gene ID	10316
Other Names	GPR66; Neuromedin-U receptor 1; NMU-R1; G-protein coupled receptor 66; G-protein coupled receptor FM-3
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GPR66. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	NMUR1 (HGNC:4518)
Function	G protein-coupled receptor for the neuropeptides neuromedin-U (NMU) and neuromedin-S (NMS) (PubMed:10899166, PubMed:15635449, PubMed:35440625). Functionally, is involved in pleiotropic roles, including regulation of feeding, energy balance, blood pressure and smooth muscle contraction (By similarity). Additionally modulates hormone release and inflammatory responses in a tissue- and receptor- dependent manner (By similarity). Mechanistically, upon ligand binding, couples to Galpha proteins, namely GNAQ/Gaq or GNA11/Ga11 and GNAI1/Gai subunits (PubMed:15331768). GNAQ/Gaq or GNA11/Ga11 coupling activatse a phospholipase C (PLC), hydrolyzing PIP2 (phosphatidylinositol 4,5- bisphosphate) into DAG (diacylglycerol) and IP3 (inositol trisphosphate) and mobilizing intracellular calcium via IP3 receptors on the endoplasmic reticulum (PubMed:10899166, PubMed:15331768). Also couples to GNAI1/Gai to inhibit forskolin-stimulated cAMP accumulation (PubMed:15331768). In pancreatic beta cells, through neuromedin U-25, regulates insulin secretion where it coupled to GNAI2 and GNAO1, resulting in reduced intracellular Ca2+ influx and cAMP levels (By similarity). Through neuromedin U-25 in Th2-type T cells, enhances type 2 cytokine secretion and modulates inflammatory responses via activation of PLC-calcineurin-MEK and PI3K signaling pathways (By similarity).
Cellular Location	Cell membrane; Multi-pass membrane protein
Tissue Location	Expressed in greatest abundance in peripheral organs, particularly in elements of the gastrointestinal and urogenital systems with highest levels in testes. In central nervous system structures express levels are much lower than those seen in peripheral organs. Within the CNS, has been detected in highest abundance in the cerebellum, dorsal root ganglia, hippocampus, and spinal cord