Anti-TPH1 (Phospho-S58) Antibody

Western blot analysis of TPH1 (Phospho-S58) expression in HEK293T (A), THP1 (B), MCF7 (C), SGC7901 (D) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 51 kD)
Immunohistochemical analysis of TPH1 (Phospho-S58) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of TPH1 (pS58) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P17752
Reactivity	Human, Mouse, Rat, Rabbit, Chicken
Host	Rabbit
Clonality	Polyclonal
Calculated MW	50985 Da

Additional Information
Gene ID	7166
Other Names	TPH; TPRH; TRPH; Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1
Target/Specificity	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S58 of human TPH1 protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~WB (1/500 - 1/1000), IHC (1/50 - 1/100)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TPH1
Synonyms	TPH, TPRH, TRPH
Function	Oxidizes L-tryptophan to 5-hydroxy-l-tryptophan in the rate- determining step of serotonin biosynthesis.
Tissue Location	[Isoform 2]: Seems to be less widely expressed than isoform 1.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.