Anti-RGS19 (Phospho-S151) Antibody

Western blot analysis of RGS19 (Phospho-S151) expression in A549 (A), H1792 (B), HepG2 (C), rat heart (D) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 24 kD)
Immunohistochemical analysis of RGS19 (Phospho-S151) staining in human stomach cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P49795
Reactivity	Human, Mouse, Rat, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	24636 Da

Additional Information
Gene ID	10287
Other Names	GAIP; GNAI3IP; Regulator of G-protein signaling 19; RGS19; G-alpha-interacting protein; GAIP
Target/Specificity	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S151 of human RGS19 protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	RGS19
Synonyms	GAIP, GNAI3IP
Function	Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G-alpha subfamily 1 members, with the order G(i)a3 > G(i)a1 > G(o)a >> G(z)a/G(i)a2. Activity on G(z)-alpha is inhibited by phosphorylation and palmitoylation of the G protein.
Cellular Location	Membrane; Lipid-anchor.
Tissue Location	Highest expression in lung. Placenta, liver and heart also express high levels of GAIP

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.