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Anti-PLC beta 3 Antibody

Rabbit polyclonal antibody to PLC beta 3

     
  • 1 - Anti-PLC beta 3 Antibody AP61288
    Western blot analysis of PLC beta 3 expression in HCT116 (A), Beas2B (B), PMVEC (C), CT26 (D) whole cell lysates.
  • 2 - Anti-PLC beta 3 Antibody AP61288
    Immunohistochemical analysis of PLC beta 3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • 3 - Anti-PLC beta 3 Antibody AP61288
    Immunofluorescent analysis of PLC beta 3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF/IC, IHC
Primary Accession Q01970
Other Accession P51432
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 138799 Da
Additional Information
Gene ID 5331
Other Names 1-phosphatidylinositol 45-bisphosphate phosphodiesterase beta-3; Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3; PLC-beta-3
Target/Specificity Recognizes endogenous levels of PLC beta 3 protein.
Dilution WB~~WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/IC (1/100 - 1/500)
IF/IC~~N/A
IHC~~WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name PLCB3 {ECO:0000303|PubMed:20966218, ECO:0000312|EMBL:AAA77683.1}
Function Catalyzes the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed:20966218, PubMed:29122926, PubMed:37991948, PubMed:9188725). Key transducer of G protein-coupled receptor signaling: activated by G(q)/G(11) G alpha proteins downstream of G protein-coupled receptors activation (PubMed:20966218, PubMed:37991948). In neutrophils, participates in a phospholipase C-activating N-formyl peptide-activated GPCR (G protein-coupled receptor) signaling pathway by promoting RASGRP4 activation by DAG, to promote neutrophil functional responses (By similarity).
Cellular Location Cytoplasm. Membrane {ECO:0000250|UniProtKB:Q99JE6}. Nucleus {ECO:0000250|UniProtKB:P51432} Note=And particulate fractions. {ECO:0000250|UniProtKB:Q99JE6}
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PLC beta 3. The exact sequence is proprietary.

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