Anti-ZNF18 Antibody

Western blot analysis of ZNF18 expression in Raw264.7 (A), SKOVCAR3 (B), MCF7 (C) whole cell lysates. (Predicted band size: 62 kD; Observed band size: 72 kD)
Immunofluorescent analysis of ZNF18 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P17022
Reactivity	Human, Mouse, Rat, Pig, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	62288 Da

Additional Information
Gene ID	7566
Other Names	HDSG1; KOX11; ZKSCAN6; ZNF535; Zinc finger protein 18; Heart development-specific gene 1 protein; Zinc finger protein 535; Zinc finger protein KOX11; Zinc finger protein with KRAB and SCAN domains 6
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ZNF18. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ZNF18
Synonyms	HDSG1, KOX11, ZKSCAN6, ZNF535
Function	May be involved in transcriptional regulation.
Cellular Location	Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00187}.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.