Anti-Carboxypeptidase O Antibody

Western blot analysis of Carboxypeptidase O expression in H1792 (A), MCF7 (B), U87MG (C), CT26 (D), C6 (E), DLD (F) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 42 kD)
Immunofluorescent analysis of Carboxypeptidase O staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q8IVL8
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	42529 Da

Additional Information
Gene ID	130749
Other Names	Carboxypeptidase O; CPO
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Carboxypeptidase O. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CPO (HGNC:21011)
Function	Carboxypeptidase which preferentially cleaves C-terminal acidic residues from peptides and proteins. Can also cleave C-terminal hydrophobic amino acids, with a preference for small residues over large residues.
Cellular Location	Apical cell membrane; Lipid-anchor, GPI-anchor
Tissue Location	Detected in enterocytes of the ileum.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.