Anti-RRH Antibody

Western blot analysis of RRH expression in MCF7 (A), PC3 (B) whole cell lysates. (Predicted band size: 37 kD; Observed band size: 37 kD)
Immunohistochemical analysis of RRH staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	O14718
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	37423 Da

Additional Information
Gene ID	10692
Other Names	Visual pigment-like receptor peropsin
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RRH. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	RRH (HGNC:10450)
Function	Opsin-family protein localized to the apical microvilli of the retinal pigment epithelium (RPE), where it may participate in retinoid exchange between photoreceptors and RPE or light-dependent retinoid processing. Although it possesses the structural features of a GPCR, no evidence for canonical G protein activation in vivo has been reported.
Cellular Location	Membrane; Multi-pass membrane protein. Apical cell membrane {ECO:0000250\|UniProtKB:O35214}; Multi-pass membrane protein. Note=Localized to the apical microvilli of RPE cells {ECO:0000250\|UniProtKB:O35214}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.