Anti-HMGXB3 Antibody

Rabbit polyclonal antibody to HMGXB3

Western blot analysis of HMGXB3 expression in A549 (A), MCF7 (B) whole cell lysates.
Immunohistochemical analysis of HMGXB3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q12766
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	141106 Da

Additional Information
Gene ID	22993
Other Names	KIAA0194; SMF; HMG domain-containing protein 3; HMG box-containing protein 3; Protein SMF
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human HMGXB3. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/50 - 1/200) IHC~~WB (1/500 - 1/1000), IHC (1/50 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt