Anti-Fetuin A Antibody

Western blot analysis of Fetuin A expression in PC12 (A), AML12 (B), HEK293T (C), H1792 (D) whole cell lysates. (Predicted band size: 39 kD; Observed band size: 59-70 kD)
Immunofluorescent analysis of Fetuin A staining in PC3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P02765
Reactivity	Human, Mouse, Rat, Bovine, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	39341 Da

Additional Information
Gene ID	197
Other Names	FETUA; Alpha-2-HS-glycoprotein; Alpha-2-Z-globulin; Ba-alpha-2-glycoprotein; Fetuin-A
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Fetuin A. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	AHSG
Synonyms	FETUA
Function	Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
Cellular Location	Secreted.
Tissue Location	Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.