Anti-Fumarase Antibody

Mouse monoclonal antibody to Fumarase

Western blot analysis of Fumarase expression in 293T (A), HepG2 (B), Hela (C), mouse brain (D), rat brain (E) whole cell lysates.
Immunofluorescent analysis of Fumarase staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P07954
Other Accession	P97807
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Calculated MW	54637 Da

Additional Information
Gene ID	2271
Other Names	Fumarate hydratase mitochondrial; Fumarase
Target/Specificity	Recognizes endogenous levels of Fumarase protein.
Dilution	WB~~WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	FH {ECO:0000303\|PubMed:27037871, ECO:0000312\|HGNC:HGNC:3700}
Function	Catalyzes the reversible stereospecific interconversion of fumarate to L-malate (PubMed:30761759). Experiments in other species have demonstrated that specific isoforms of this protein act in defined pathways and favor one direction over the other (Probable).
Cellular Location	[Isoform Mitochondrial]: Mitochondrion
Tissue Location	Expressed in red blood cells; underexpressed in red blood cells (cytoplasm) of patients with hereditary non-spherocytic hemolytic anemia of unknown etiology.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.