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CK7 Monoclonal Antibody(12D7)

Immunofluorescence analysis of human-liver tissue. 1,CK7 Monoclonal Antibody(12D7)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of 1) Hela, 2) Mouse Kidney, 3) Mouse Brain, diluted at 1:2000.
IHC staining of human lung cancer tissue, diluted at 1:200.
1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IF, ICC, WB, IHC-P, IP
Primary Accession	P08729
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal

Additional Information
Other Names	KRT7; SCL; Keratin, type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7
Dilution	IF~~IF: 1:50-200 WB: 1:1000-2000 IHC: 1:200 IP:1:200 ICC~~N/A WB~~IF: 1:50-200 WB: 1:1000-2000 IHC: 1:200 IP:1:200 IHC-P~~IF: 1:50-200 WB: 1:1000-2000 IHC: 1:200 IP:1:200 IP~~N/A
Format	PBS, pH 7.4, containing 0.09% (W/V) sodium azide as Preservative and 50% Glycerol.
Storage Conditions	-20℃

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

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¥ 1,520.00

Cat# AP63308

Size:

50 µl

100 µl

Price:

¥ 1,520.00

¥ 2,050.00

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Availability: 3-5days

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CK7 Monoclonal Antibody(12D7)

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