|Application ||WB, IHC-P, FC, E|
|Other Accession||Q68FR9, P57776, Q4R3D4|
|Predicted||Monkey, Mouse, Rat|
|Calculated MW||31122 Da|
|Other Names||Elongation factor 1-delta, EF-1-delta, Antigen NY-CO-4, EEF1D, EF1D|
|Target/Specificity||This EEF1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EEF1D.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EEF1D Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1- alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.|
|Cellular Location||Isoform 2: Nucleus|
|Tissue Location||Isoform 2 is specifically expressed in brain, cerebellum and testis|
Provided below are standard protocols that you may find useful for product applications.
EEF1D is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs.
Yang,S., BMC Cancer 7, 211 (2007)
Mulner-Lorillon,O., J. Biol. Chem. 269 (31), 20201-20207 (1994)