FAM3B Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-FAM3B Antibody (N-term) at 1:1000 dilution + Daudi whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with FAM3B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
FAM3B Antibody (N-term) (Cat. #AP6891a) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, FC, WB, E
Primary Accession	P58499
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	25982 Da
Antigen Region	24-51 aa

Additional Information
Gene ID	54097
Other Names	Protein FAM3B, Cytokine-like protein 2-21, Pancreatic-derived factor, PANDER, FAM3B, C21orf11, C21orf76
Target/Specificity	This FAM3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-51 amino acids from the N-terminal region of human FAM3B.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	FAM3B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	FAM3B
Synonyms	C21orf11, C21orf76
Function	Induces apoptosis of alpha and beta cells in a dose- and time-dependent manner.
Cellular Location	Secreted. Note=Present in insulin secretory granules and likely cosecreted with insulin. Localized in discrete vesicular and perinuclear structure
Tissue Location	Highly expressed in the pancreas. Also found in the colon, kidney, prostate, small intestine and testis

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.