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FAM3B Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - FAM3B Antibody (N-term) AP6891a
    Anti-FAM3B Antibody (N-term) at 1:1000 dilution + Daudi whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - FAM3B Antibody (N-term) AP6891a
    Formalin-fixed and paraffin-embedded human colon carcinoma reacted with FAM3B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - FAM3B Antibody (N-term) AP6891a
    FAM3B Antibody (N-term) (Cat. #AP6891a) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB, E
Primary Accession P58499
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 25982 Da
Additional Information
Gene ID 54097
Other Names Protein FAM3B, Cytokine-like protein 2-21, Pancreatic-derived factor, PANDER, FAM3B, C21orf11, C21orf76
Target/Specificity This FAM3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-51 amino acids from the N-terminal region of human FAM3B.
Dilution WB~~1:1000
IHC-P~~1:100~500
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFAM3B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FAM3B
Synonyms C21orf11, C21orf76
Function Induces apoptosis of alpha and beta cells in a dose- and time-dependent manner.
Cellular Location Secreted. Note=Present in insulin secretory granules and likely cosecreted with insulin. Localized in discrete vesicular and perinuclear structure
Tissue Location Highly expressed in the pancreas. Also found in the colon, kidney, prostate, small intestine and testis
Research Areas

BACKGROUND

FAM3B has delayed effects on beta-cell function, inhibiting basal insulin secretion from a beta-cell line in a dose-dependent manner.

REFERENCES

Yang,J., et.al., Biochemistry 44 (34), 11342-11352 (2005)

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