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SAR1A Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - SAR1A Antibody (Center) AP6915c
    Western blot analysis of lysates from 293,HT1080 cell line,human placenta,mouse liver and lung tissue (from left to right),using SAR1A Antibody (Center)(Cat. #AP6915c).AP6915c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
  • 14 - SAR1A Antibody (Center) AP6915c
    Formalin-fixed and paraffin-embedded human lung carcinoma with SAR1A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - SAR1A Antibody (Center) AP6915c
    Flow cytometric analysis of NCI-H292 cells using SAR1A Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB, E
Primary Accession Q9NR31
Other Accession Q52NJ3, P36536, Q3T0D7
Reactivity Human, Mouse
Predicted Bovine, Mouse, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 22367 Da
Antigen Region 122-149 aa
Additional Information
Gene ID 56681
Other Names GTP-binding protein SAR1a, COPII-associated small GTPase, SAR1A, SAR1, SARA, SARA1
Target/Specificity This SAR1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-149 amino acids from the Central region of human SAR1A.
Dilution IHC-P~~1:100~500
FC~~1:10~50
WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSAR1A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SAR1A (HGNC:10534)
Synonyms SAR1, SARA, SARA1
Function Small GTPase that cycles between an active GTP-bound and an inactive GDP-bound state and mainly functions in vesicle-mediated endoplasmic reticulum (ER) to Golgi transport. The active GTP-bound form inserts into the endoplasmic reticulum membrane where it recruits the remainder of the coat protein complex II/COPII. The coat protein complex II assembling and polymerizing on endoplasmic reticulum membrane is responsible for both the sorting of cargos and the deformation and budding of membranes into vesicles destined to the Golgi (PubMed:23433038, PubMed:32358066, PubMed:36369712). The GTPase activity of SAR1 by controlling the timing of COPII budding regulates the size of the formed vesicles and is important for cargo selection depending on their size (PubMed:32358066). Together with SEC16A, forms the organized scaffold defining endoplasmic reticulum exit sites (ERES), some specific domains of the endoplasmic reticulum where COPII vesicles form (PubMed:17005010). In addition to its role in vesicle trafficking, can also function as a leucine sensor regulating TORC1 signaling and more indirectly cellular metabolism, growth and survival. In absence of leucine, interacts with the GATOR2 complex via MIOS and inhibits TORC1 signaling. The binding of leucine abrogates the interaction with GATOR2 and the inhibition of the TORC1 signaling. This function is completely independent of the GTPase activity of SAR1B (PubMed:34290409).
Cellular Location Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Cytoplasm, cytosol. Lysosome membrane. Note=Active at endoplasmic reticulum exit sites (ERES) where it inserts into the membrane and recruits the remainder of the coat protein complex II/COPII (PubMed:23433038, PubMed:32358066). Upon leucine deprivation, associates with lysosomal membranes to repress TORC1 signaling (Probable)
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

SAR1A is involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity) and required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).

REFERENCES

Morgan,A.R., et.al., Am. J. Med. Genet. B Neuropsychiatr. Genet. 144B (6), 762-770(2007)

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