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TGM4 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-TGM4 Antibody (Center) at 1:1000-1:2000 dilution Lane 1: PC-3 whole cell lysateLane 2: Hela whole cell lysate Lane 3: LNCap whole cell lysate Lane 4: CCRF-CEM whole cell lysate Lane 5: SH-SY5Y whole cell lysate Lane 6: Mouse prostate whole tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 77 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of TGM4 Antibody (Center) (Cat. #AP6918c) in CEM cell line lysates (35ug/lane). TGM4 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human prostate carcinoma with TGM4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of CEM cells using TGM4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	P49221
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	77145 Da
Antigen Region	135-164 aa

Additional Information
Gene ID	7047
Other Names	Protein-glutamine gamma-glutamyltransferase 4, Fibrinoligase, Prostate transglutaminase, Prostate-specific transglutaminase, Transglutaminase P, TG(P), TGP, TGase P, Transglutaminase-4, TGase-4, TGM4
Target/Specificity	This TGM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the Central region of human TGM4.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	TGM4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	TGM4
Function	Associated with the mammalian reproductive process. Catalyzes the cross-linking of proteins and the conjugation of polyamines to specific proteins in the seminal tract.
Tissue Location	Prostate.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

TGM4is associated with the mammalian reproductive process and catalyzes the cross-linking of proteins and the conjugation of polyamines to specific proteins in the seminal tract.

REFERENCES

Jiang,W.G., et.al., Microvasc. Res. 77 (2), 150-157 (2009)

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¥ 1,250.00

Cat# AP6918c

Size:

50 µl

100 µl

200 µl

400 µl

Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

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Availability: In Stock

询价

TGM4 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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