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Cyclin E1 Polyclonal Antibody

     
  • 1 - Cyclin E1 Polyclonal Antibody AP69359
    Immunofluorescence analysis of rat-spleen tissue. 1,Cyclin E1 Polyclonal Antibody(red) was diluted at 1:200(4°,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 1 - Cyclin E1 Polyclonal Antibody AP69359
    Western Blot analysis of various cells using Cyclin E1 Polyclonal Antibody diluted at 1:500
  • 1 - Cyclin E1 Polyclonal Antibody AP69359
    Western Blot analysis of MCF7 cells using Cyclin E1 Polyclonal Antibody diluted at 1:500
  • 14 - Cyclin E1 Polyclonal Antibody AP69359
    Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
  • 14 - Cyclin E1 Polyclonal Antibody AP69359
    Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
  • 14 - Cyclin E1 Polyclonal Antibody AP69359
    Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF
Primary Accession P24864
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 47077 Da
Additional Information
Gene ID 898
Other Names CCNE1; CCNE; G1/S-specific cyclin-E1
Dilution WB~~Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
IHC-P~~Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
IF~~1:50~200
Format Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions-20℃
Protein Information
Name CCNE1
Synonyms CCNE
Function Essential for the control of the cell cycle at the G1/S (start) transition.
Cellular Location Nucleus.
Tissue Location Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Essential for the control of the cell cycle at the G1/S (start) transition.

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