癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
NEK8 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | Q86SG6 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 74806 Da |
| Antigen Region | 573-604 aa |
| Gene ID | 284086 |
|---|---|
| Other Names | Serine/threonine-protein kinase Nek8, Never in mitosis A-related kinase 8, NimA-related protein kinase 8, Nima-related protein kinase 12a, NEK8, JCK, NEK12A |
| Target/Specificity | This NEK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 573-604 amino acids from the C-terminal region of human NEK8. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NEK8 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NEK8 |
|---|---|
| Synonyms | JCK, NEK12A |
| Function | Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia (PubMed:37598857). Plays a role in organogenesis, and is involved in the regulation of the Hippo signaling pathway (PubMed:26967905). |
| Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q91ZR4}. Cell projection, cilium. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, cilium axoneme. Note=Predominantly cytoplasmic Localizes to the proximal region of the primary cilium and is not observed in dividing cells. {ECO:0000250|UniProtKB:Q91ZR4} |
| Tissue Location | Highest expression in thyroid, adrenal gland and skin. Low levels in spleen, colon and uterus. Overexpressed in breast tumors, with highest expression in infiltrating ductal carcinomas and moderate levels in mucinous adenocarcinoma |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
NEK8 is a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans. The encoded protein may play a role in cell cycle progression from G2 to M phase. Mutations in the related mouse gene are associated with a disease phenotype that closely parallels the juvenile autosomal recessive form of polycystic kidney disease in humans.
REFERENCES
Valkova,N. et al. Mol. Cell Proteomics 4 (7), 1009-1018 (2005)
Bowers,A.J. et al. Gene 328, 135-142 (2004)
Holland,P.M. et al. J. Biol. Chem. 277 (18), 16229-16240 (2002)
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