癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
NEK8 Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF, E |
|---|---|
| Primary Accession | Q86SG6 |
| Other Accession | Q86SG6 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 74806 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human NEK8 |
| Epitope Specificity | 101-200/692 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the protein kinase superfamily. NEK Ser/Thrprotein kinase family. NIMA subfamily.Contains 1 protein kinase domain.Contains 5 RCC1 repeats. |
| SUBUNIT | Interacts with PKD2; may regulate PKD2 targeting to thecilium (By similarity). |
| DISEASE | Defects in NEK8 are the cause of nephronophthisis type 9(NPHP9) [MIM:613824]. NPHP9 is an autosomal recessive disorderresulting in end-stage renal disease. It is a progressivetubulo-interstitial kidney disorder histologically characterized bymodifications of the tubules with thickening of the basementmembrane, interstitial fibrosis and, in the advanced stages,medullary cysts. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | Required for renal tubular integrity. Tissue specificity: Highest expression in thyroid, adrenal gland and skin. Low levels in spleen, colon and uterus. Overexpressed in breast tumors, with highest expression in infiltrating ductal carcinomas and moderate levels in mucinous adenocarcinoma. |
| Gene ID | 284086 |
|---|---|
| Other Names | JCK; NEK12A; NPHP9; PKD8; RHPD2; NEK8_HUMAN; NEK8; Never in mitosis A-related kinase 8 (NimA-related protein kinase 8); Nima-related protein kinase 12a; 2.7.11.1; NIMA related kinase 8; NIMA (never in mitosis gene a)- related kinase 8 |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | NEK8 |
|---|---|
| Synonyms | JCK, NEK12A |
| Function | Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia (PubMed:37598857). Plays a role in organogenesis, and is involved in the regulation of the Hippo signaling pathway (PubMed:26967905). |
| Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q91ZR4}. Cell projection, cilium. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, cilium axoneme. Note=Predominantly cytoplasmic Localizes to the proximal region of the primary cilium and is not observed in dividing cells. {ECO:0000250|UniProtKB:Q91ZR4} |
| Tissue Location | Highest expression in thyroid, adrenal gland and skin. Low levels in spleen, colon and uterus. Overexpressed in breast tumors, with highest expression in infiltrating ductal carcinomas and moderate levels in mucinous adenocarcinoma |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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