DFNA5 Polyclonal Antibody

Western Blot analysis of extracts from rat stomach, 293 cells, using DFNA5 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	O60443
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	54555 Da

Additional Information
Gene ID	1687
Other Names	DFNA5; ICERE1; Non-syndromic hearing impairment protein 5; Inversely correlated with estrogen receptor expression 1; ICERE-1
Dilution	WB~~Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Format	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions	-20℃

Protein Information
Name	GSDME {ECO:0000303\|PubMed:28459430, ECO:0000312\|HGNC:HGNC:2810}
Function	[Gasdermin-E]: Precursor of a pore-forming protein that converts non-inflammatory apoptosis to pyroptosis (PubMed:27281216, PubMed:28459430, PubMed:33852854, PubMed:35594856, PubMed:36607699). This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-E, N-terminal) binds to membranes and forms pores, triggering pyroptosis (PubMed:28459430).
Cellular Location	[Gasdermin-E, N-terminal]: Cell membrane; Multi-pass membrane protein {ECO:0000250\|UniProtKB:Q5Y4Y6}
Tissue Location	Expressed in cochlea (PubMed:9771715). Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta (PubMed:9771715).