RhCG Polyclonal Antibody

Western Blot analysis of extracts from Jurkat, K562 cells, using RhCG Polyclonal Antibody.. Secondary antibody was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P
Primary Accession	Q9UBD6
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	53179 Da

Additional Information
Gene ID	51458
Other Names	RHCG; C15orf6; CDRC2; PDRC2; RHGK; Ammonium transporter Rh type C; Rh glycoprotein kidney; Rhesus blood group family type C glycoprotein; Rh family type C glycoprotein; Rh type C glycoprotein; Tumor-related protein DRC2
Dilution	WB~~Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/40000. Not yet tested in other applications. IHC-P~~Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/40000. Not yet tested in other applications.
Format	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions	-20℃

Protein Information
Name	RHCG
Synonyms	C15orf6, CDRC2, PDRC2, RHGK
Function	Ammonium transporter involved in the maintenance of acid-base homeostasis. Transports ammonium and its related derivative methylammonium across the plasma membrane of epithelial cells likely contributing to renal transepithelial ammonia transport and ammonia metabolism. Postulated to primarily mediate an electroneutral bidirectional transport of NH3 ammonia species according to a mechanism that implies interaction of an NH4(+) ion with acidic residues of the pore entry followed by dissociation of NH4(+) into NH3 and H(+). As a result NH3 transits through the central pore and is protonated on the extracellular side reforming NH4(+) (PubMed:11062476, PubMed:14761968, PubMed:15929723, PubMed:16477434, PubMed:16580862, PubMed:24077989). May act as a CO2 channel providing for renal acid secretion (PubMed:24077989).
Cellular Location	Cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Note=Also detected at the basolateral membrane and in subapical vesicles.
Tissue Location	Expressed in brain, testis, placenta, pancreas, esophagus and prostate. Expressed in squamous epithelial tissues (at protein level). Expressed in kidney.